Yes-ish.

There's a lot of different and overlapping programs in play here. I do not believe that autonomous F-16s are intended to be operational assets. That said...

VENOM - program that shows you can bolt on autonomous flight control into a standard F-16. Previously, the X-62A (a one-off prototype based F-16) demonstrated autonomous dogfighting back in 2023.

VENOM provides USAF/DARPA with a lot of high performance autonomous platforms (bolt-on F-16s) that they can then use to develop and validate software, tactics and training for actually fighting with autonomous systems. This would presumably be critical for getting the most of the CCA (aka loyal wingman) programs currently in development.

The AIR program referenced is basically "how do we get autonomous systems to control and fight effectively as a team (presumably arbitrary mixture of manned and unmanned systems) in a beyond visual range fight".

The ACE program referenced was basically "how do we get autonomous platforms to dogfight" with related questions like "how can we augment the human pilot in a dogfight - ie, how can we hand off the actual flying and execution of manoeuvres, and using all of the sensors to autonomous systems, and let the pilot choose actions, instead of hang flying the actions".