What is the accuracy in this ? Aka if I run the experiment 10 times how many differences will i get? I don’t have a physical sense on what would be a good number.

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myhf a day ago | parent | next [-]

You would get a lot of differences, but the errors would cancel each other out with enough depth of coverage.

This technology's baseline accuracy is around 95% per base, so 10x reads of every segment in the sample would give >99% accuracy for each base after aligning the reads with each other.

https://en.wikipedia.org/wiki/Coverage_(genetics)

	▲	Jules-Bertholet a day ago \| parent [-]
		> so 10x reads of every segment in the sample would give >99% accuracy for each base after aligning the reads with each other This assumes random errors, which IIRC isn't the case for Oxford Nanopore.

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Jules-Bertholet a day ago | parent | prev [-]

Oxford Nanopore unfortunately has a high error rate (3-5%) compared to other sequencing technologies. And the errors are non-random

	▲	mbeavitt 19 hours ago \| parent \| next [-]
		This is simply not true, advances in basecalling and using Duplex give you >Q25
	▲	Gethsemane 20 hours ago \| parent \| prev [-]
		For most DNA it now sits at ~Q20 (1%) which is a big improvement - there was also some hacky stuff like HERRO and duplex sequencing which improved it substantially. For routine genome sequencing it's definitely suitable, and the long-read advantage helps resolve some of those trickier regions