| ▲ | zulko 2 hours ago | |
Possibly not what you're asking for, but I wrote a generally-accessible intro to why it can be tricky to assemble many DNA fragments with "Golden Gate Assembly", a mainstream method which relies on short sequence overhangs. The Sidewinder method discussed in this thread aims to solve that "short overhang" problem. https://zulko.github.io/bricks_and_scissors/posts/overhangs/ | ||