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codesnik 5 hours ago

pcr amplifies all sequences, correct or wrong, no? and as I understand it, it works on short snippets the best.

biophysboy 4 hours ago | parent [-]

It amplifies sequences that contain the two primer sequences on each end of the target. So if you had synthesized sequence XYZ with some mistakes like YZX, then you could target X and Z and purify.

You're correct that PCR has a limited max length, but it is longer and cheaper than vanilla DNA synthesis.

bookofjoe 2 hours ago | parent [-]

Kary B. Mullis Nobel Prize lecture Nobel Lecture, December 8, 1993

The Polymerase Chain Reaction

https://www.nobelprize.org/prizes/chemistry/1993/mullis/lect...