| ▲ | codesnik 5 hours ago | |||||||
pcr amplifies all sequences, correct or wrong, no? and as I understand it, it works on short snippets the best. | ||||||||
| ▲ | biophysboy 4 hours ago | parent [-] | |||||||
It amplifies sequences that contain the two primer sequences on each end of the target. So if you had synthesized sequence XYZ with some mistakes like YZX, then you could target X and Z and purify. You're correct that PCR has a limited max length, but it is longer and cheaper than vanilla DNA synthesis. | ||||||||
| ||||||||