Interesting guide to the Whole Slide Images (WSI) format. The surprising thing for me is that compression is used, and they note does not affect use in diagnostics.

Back in the day we used TIFF for a similar application (X-ray detector images).

yread 3 hours ago | parent [-]

Digital pathology are just a lot bigger than radiology, we regularly see slides 500k x 500k pixels.

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el_pa_b an hour ago | parent [-]

Yes, they can be huge, and for modalities like multiplex immunofluorescence with up to 20 channels, you're often dealing with very faint proteomic signals. Preserving that signal is critical, and compression can destroy it quickly.

	▲	yread an hour ago \| parent [-]
		CODEX can do up to 120 channels I think. They are also 16/32bit. They are usually just deflated