| ▲ | anfractuosity 8 hours ago | |||||||||||||
Sounds fascinating, do you have any documentation on how you modify the yeast? | ||||||||||||||
| ▲ | koeng 8 hours ago | parent [-] | |||||||||||||
I really need to do a write-up. I kinda just whip up the easiest path and do it. For example for the grape, I needed to knock out some tryptophan synthesis genes so I could redirect the bioflux. Problem is that in bakers yeast they have a whole buncha copies of their chromosomes, so I had to knock out one of the genes and replace it with a different gene from grapes. Did that with a quick lil CRISPR switch. Had to electroporate tho because the transformation rates on wild/bakers/non-lab yeast are so garbage | ||||||||||||||
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