I'm not sure if this is true, or for how long it has been true. I rooted my company phone (Samsung Galaxy S4), removed the crapware, and un-rooted it so that it could join the corporate network. This was a long time ago.

Rooting certainly blows the Vault eFuse. Knox Vault, etc. are newer than the S4 (Knox Vault was introduced in the S21).

For removing bloatware from the user partition you don't need to root, adb or the universal android debloater will do.